SLH#01: Biomedical Applications of Synchrotron Radiation Micro and Nano Imaging

Thursday 8 Jul 2021, 15:00 → 18:00 Asia/Tehran

985 0323 0648 (Zoom Meeting ID:)

Ehsan Salimi (IPM), Sam BAYAT (CHU Grenoble Alpes), Sharmin Kharrazi (TUMS)

Join Zoom Meeting* https://zoom.us/j/98503230648?pwd=UUtYUTVoV21yTE5xUWhibWtSR1k2Zz09 Meeting ID: 985 0323 0648 Passcode: TUMS
Webinar: Biomedical Applications of Synchrotron Radiation Micro and Nano Imaging The first session from "Synchrotron Light on Health Sciences" webinar series		وبینار: کاربردهای تصویربرداری سنکروترونیِ میکرو و نانو در زیست‌پزشکی نخستین قسمت از مجموعه وبینارهای «کاربردهای تابش سنکروترونی در علوم سلامت»
This webinar series will focus on the latest applications of synchrotron light in various diciplines of health sciences The first session from this series will be held on Thursday, July 8th, 2021, 10:30-13:30 (GMT) which is dedicated to Micro & Nano Imaging and consists of four talks delivered by experienced scientists from CHU Grenoble-Alpes and CNRS, France. These webinars are jointly held by the School of Advanced Technologies in Medicine (SATiM) in Tehran University of Medical Sciences, and the Iranian Light Source Facility (ILSF) in Institute for Research in Fundamental Sciences (IPM), Iran. Registration is free for all medical students and scientists around the world and all participants will be given Certification of Attendance.		این مجموعه به روش‌های مبتنی بر تابش سنکروترون و دستاوردهای آن در زمینه‌های گوناگون علوم پزشکی و سلامت می‌پردازد. این وبینارها توسط دانشکده فناوری‌های نوین پزشکی (دانشگاه علوم پزشکی تهران) با همکاری چشمه نور ایران برگزار می‌شود. نخستین وبینار در تاریخ پنج‌شنبه، ۱۷ تیرماه ۱۴۰۰ ساعت ۱۵ تا ۱۸ با محور «تصویربرداری میکرو و نانو» و با سخنرانی متخصصین باتجربه از مرکز بیمارستانی دانشگاه گرنوبل-آلپ و نیز مرکز ملی پژوهش‌های علمی در فرانسه برگزار می‌شود. مخاطبین این مجموعه دانشجویان و پژوهشگران علوم پزشکی، متخصصین تصویربرداری و دانشگرانی در زمینه‌های مرتبط هستند. نام‌نویسی در این وبینار تخصصی برای علاقه‌مندان رایگان است و به شرکت‌کنندگان گواهی حضور داده خواهد شد.
Date & Time:	8 July 2021 10:30 - 13:30 GMT	۱۷ تیرماه ۱۴۰۰ ۱۵:۰۰-۱۸:۰۰ به وقت ایران	تاريخ و زمان:
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Poster Announcement Poster

15:00 Short Video

15:05 Oppening

1 Shedding Light on Regional Lung Structure & Function with Synchrotron Radiation

Elucidating the 3D structure and real-time function of the lung at small length scales in vivo, is one of the most challenging applications of synchrotron radiation in biomedical imaging. Dynamic measurements allowing the study of regional lung function, with methods using synchrotron radiation such as K-edge subtraction imaging are crucial for better understanding of phenomena such as gas transport and exchange, adverse effects of mechanical ventilation on the lung and strategies to prevent them, aerosol transport and deposition, among other applications. On the other hand, the coherence of synchrotron beams allows phase-contrast imaging of the poorly radiation-absorbing lung tissue, allowing enhancement of structural details. Real-time imaging of lung function is highly challenging at small length-scales. There is currently a trade-off between spatial and temporal resolutions, and both are difficult to achieve simultaneously. In vivo synchrotron radiation imaging also faces limitations due to radiation dose. Improvements in the available detector technology, allowing for significant gains in spatial resolution, efficiency, dynamic range, energy resolution as well as temporal resolution will be crucial for overcoming these limitations. Examples of in vivo synchrotron imaging in investigating models of lung diseases will be discussed.

Speaker: Prof. Sam BAYAT (Univerisité de Grenoble Alpes)

15:40 Q&A

2 X-Ray Phase Contrast Imaging for Biomedicine

Since the seminal work of Roentgen, conventional X-ray imaging is based on the same physical phenomenon: the absorption of light by the tissues. The refraction index of light element materials can be a thousand times greater than its counterpart the absorption factor for the wavelength in radiology. In this talk we will explore the interest of using refraction of tissues at the organ and the cell scale.

Speaker: Dr Emmanuel Brun (Inserm UA07)

16:20 Q&A

16:25 Short Video

3 High-Resolution Quantitative Label-Free Microscopy: Probing the Nano-Bio-World

Today, we know how the nano-structures and the process happening at the nanometric scale affect the macroscopic behaviour of biological systems. Therefore, we need to be able to image the biological sample down to the nanometric length scale, which is a big challenge. In some cases, we can tag, stain or label the system of interest, but in other case, this is not possible and label-free imaging is necessary. In this talk, I will give a brief overview of how to carry out X-ray nanoimaging of biological systems.

Speaker: Dr Julio Cesar da Silva (Institut Néel CNRS)

17:00 Q&A

17:05 Group Photo

4 Unveiling the Chemical Landscape of Cells Using Synchrotron X-Ray Nano-Probe

Several essential metal ions participate in the control of numerous metabolic and signaling pathways, but their rich coordination chemistry and redox properties confer them a propensity to randomly coordinate and catalytically react inside the cell with protein sites other than those tailored for that purpose. A number of sophisticated networks of trafficking pathways are available to tightly regulate their uptake, intracellular transport and compartmentalization, and to avoid their toxic side effects. Cutting-edge technique providing quantitative imaging for detailed study of elemental homeostasis or the intracellular distribution of metal-based drugs at biologically relevant concentration in a label-free fashion is highly desirable. The synchrotron X-ray fluorescence (XRF) nanoprobe as developed today provide the required sensitivity and spatial resolution to elucidating the 2D and 3D distribution, concentration of elements particularly metals inside entire cells at the organelle level. The new state-of-the-art Nano-Imaging beamline ID16A-NI at ESRF offers unique capabilities for X-ray imaging at nanometer scale at high energies (~30nm at 17kev) [1]. It is particularly well suited for the investigation of biological samples at high spatial resolution, e.g. combined hard X-ray phase imaging and XRF detection and quantification of trace elements [2]. A critical issue is to best preserve the structural and chemical integrity of the cells. As it has been demonstrated in electron microscopy or recently for synchrotron 3D-XRF [3], a cryogenic workflow including cryo-immobilization of the cell and cryotransfer to a cryo-scanning stage allow an optimal elemental preservation at subcellular level as close as possible to their native state. In this work, we will illustrate the unique capabilities of this techniques to provide insight in the intracellular targeting of new organometallic drugs, i.e osmocifen [3] on breast cancer cells but also on the fate of metal-based nanoparticles on cancer cells. We will try to underline the importance of correlative sub-cellular imaging for better understanding of the role of metals in cells. References: [1] J. C. Da Silva et al, Optica 2017, 4, 492; [2] Bohic S. et al., Journal of Structural Biology, 2012, 177, 248; [3] Fus F. et al, Angwendte Chemie. 2019, doi: 10.1002/anie.201812336

Speaker: Dr Sylvain Bohic (Inserm UA07)

17:40 Q&A / Open Discussion